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myc ddk flag  (OriGene)


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    Structured Review

    OriGene myc ddk flag
    Myc Ddk Flag, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myc+ddk+flag/NCF2+(NM_000433)+Human+Tagged+ORF+Clone/pmc12936047-49-19-24
    Average 94 stars, based on 2 article reviews
    myc ddk flag - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Expressing:

    Article Title: Golgi-localized Ring Finger Protein 121 is necessary for MYCN-driven neuroblastoma tumorigenesis
    Article Snippet: Cell density was adjusted so that for all assays, 80–90% confluency would be achieved by the endpoint of the assay in the control condition. siRNA’s used were siRNF121-1: GAAAUGGUCCUCAUCCUCA (Dharmacon: J-007011-06) and siRNF121-4: CCAUAGGGUUCUACAGCGA (Dharmacon: J-007011-08) or non-targeting control siRNAs (ONTARGETplus siRNA, #D-001810-0X, Dharmacon). .. Expression plasmids of pCMV6-Empty Vector and pCMV6-hRNF121 wild type (WT) with Myc-DDK (FLAG) at c-terminus were purchased from Origene (Origene RC211747). pCMV6-hRNF121(M158R), pCMV6-hRNF121-M1, pCMV6-hRNF121-M2, pCMV6-hRNF121-M3 and pCMV6-hRNF121-M4 with Myc-DDK(FLAG) at c-terminus were subcloned and mutagenesis were performed by Genescript based on RC211747 RNF121 sequence (Genescript). .. All siRNA or plasmid transfections were conducted with Lipofectamine 2000 according to manufacturer’s instructions (Life Technologies) at 20–40 nM for siRNA or 1–2 μg of plasmid DNA.

    Article Title: NCF2 facilitates M2 macrophage polarization in glioblastoma through activation of the notch1–osteopontin axis
    Article Snippet: .. A mammalian expression plasmid encoding the full-length open reading frame (ORF) of human NCF2 ( NM_000433 ) tagged with Myc-DDK (FLAG) was obtained from OriGene (catalog no. RC200704, Rockville, MD, USA). .. For RNA interference, pre-designed Silencer Select small interfering RNAs (siRNAs) targeting human NCF2 , along with a non-targeting negative control siRNA (#4390847), were purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Article Title: Mutational and functional analysis of Glucose transporter I deficiency syndrome.
    Article Snippet: Molecular Genetics and Metabolism xxx (2015) xxx–xxx YMGME-05940; No. of pages: 6; 4C: 3, 4, 5 Contents lists available at ScienceDirect Molecular Genetics and Metabolism j ourna l homepage: www.e lsev ie r .com/ locate /ymgme Mutational and functional analysis of Glucose transporter I deficiency syndrome Sachie Nakamura a, Hitoshi Osaka a,⁎, Shinichi Muramatsu b,c, Shiho Aoki a, Eriko F. Jimbo a, Takanori Yamagata a a Department of Pediatrics, Jichi Medical University, Tochigi, Japan b Division of Neurology, Jichi Medical University, Tochigi, Japan c Center for Gene and Cell Therapy, The Institute of Medical Science, The University of Tokyo, Japan ⁎ Corresponding author at: Dept. of Pediatrics, Jichi Me Shimotsuke-shi, Tochigi 329-0498, Japan.. E-mail address: hosaka@jichi.ac.jp (H. Osaka). http://dx.doi.org/10.1016/j.ymgme.2015.08.006 1096-7192/© 2015 Elsevier Inc. All rights reserved.. Please cite this article as: S. Nakamura, et al., (2015), http://dx.doi.org/10.1016/j.ymgme.2 a b s t r a c t a r t i c l e i n f o Article history: Received 8 July 2015 Received in revised form 9 August 2015 Accepted 9 August 2015 Available online xxxx Keywords: Glucose transporter I deficiency syndrome (GLUT1-DS) GLUT1 SLC2A1 Functional assay 2-deoxyglucose uptake Objective: We investigated a correlation between a mutation in the SLC2A1 gene and functional disorders in Glucose transporter I deficiency syndrome (GLUT1DS).

    Mutagenesis:

    Article Title: Golgi-localized Ring Finger Protein 121 is necessary for MYCN-driven neuroblastoma tumorigenesis
    Article Snippet: Cell density was adjusted so that for all assays, 80–90% confluency would be achieved by the endpoint of the assay in the control condition. siRNA’s used were siRNF121-1: GAAAUGGUCCUCAUCCUCA (Dharmacon: J-007011-06) and siRNF121-4: CCAUAGGGUUCUACAGCGA (Dharmacon: J-007011-08) or non-targeting control siRNAs (ONTARGETplus siRNA, #D-001810-0X, Dharmacon). .. Expression plasmids of pCMV6-Empty Vector and pCMV6-hRNF121 wild type (WT) with Myc-DDK (FLAG) at c-terminus were purchased from Origene (Origene RC211747). pCMV6-hRNF121(M158R), pCMV6-hRNF121-M1, pCMV6-hRNF121-M2, pCMV6-hRNF121-M3 and pCMV6-hRNF121-M4 with Myc-DDK(FLAG) at c-terminus were subcloned and mutagenesis were performed by Genescript based on RC211747 RNF121 sequence (Genescript). .. All siRNA or plasmid transfections were conducted with Lipofectamine 2000 according to manufacturer’s instructions (Life Technologies) at 20–40 nM for siRNA or 1–2 μg of plasmid DNA.

    Sequencing:

    Article Title: Golgi-localized Ring Finger Protein 121 is necessary for MYCN-driven neuroblastoma tumorigenesis
    Article Snippet: Cell density was adjusted so that for all assays, 80–90% confluency would be achieved by the endpoint of the assay in the control condition. siRNA’s used were siRNF121-1: GAAAUGGUCCUCAUCCUCA (Dharmacon: J-007011-06) and siRNF121-4: CCAUAGGGUUCUACAGCGA (Dharmacon: J-007011-08) or non-targeting control siRNAs (ONTARGETplus siRNA, #D-001810-0X, Dharmacon). .. Expression plasmids of pCMV6-Empty Vector and pCMV6-hRNF121 wild type (WT) with Myc-DDK (FLAG) at c-terminus were purchased from Origene (Origene RC211747). pCMV6-hRNF121(M158R), pCMV6-hRNF121-M1, pCMV6-hRNF121-M2, pCMV6-hRNF121-M3 and pCMV6-hRNF121-M4 with Myc-DDK(FLAG) at c-terminus were subcloned and mutagenesis were performed by Genescript based on RC211747 RNF121 sequence (Genescript). .. All siRNA or plasmid transfections were conducted with Lipofectamine 2000 according to manufacturer’s instructions (Life Technologies) at 20–40 nM for siRNA or 1–2 μg of plasmid DNA.

    Plasmid Preparation:

    Article Title: NCF2 facilitates M2 macrophage polarization in glioblastoma through activation of the notch1–osteopontin axis
    Article Snippet: .. A mammalian expression plasmid encoding the full-length open reading frame (ORF) of human NCF2 ( NM_000433 ) tagged with Myc-DDK (FLAG) was obtained from OriGene (catalog no. RC200704, Rockville, MD, USA). .. For RNA interference, pre-designed Silencer Select small interfering RNAs (siRNAs) targeting human NCF2 , along with a non-targeting negative control siRNA (#4390847), were purchased from Thermo Fisher Scientific (Waltham, MA, USA).

    Article Title: Mutational and functional analysis of Glucose transporter I deficiency syndrome.
    Article Snippet: Molecular Genetics and Metabolism xxx (2015) xxx–xxx YMGME-05940; No. of pages: 6; 4C: 3, 4, 5 Contents lists available at ScienceDirect Molecular Genetics and Metabolism j ourna l homepage: www.e lsev ie r .com/ locate /ymgme Mutational and functional analysis of Glucose transporter I deficiency syndrome Sachie Nakamura a, Hitoshi Osaka a,⁎, Shinichi Muramatsu b,c, Shiho Aoki a, Eriko F. Jimbo a, Takanori Yamagata a a Department of Pediatrics, Jichi Medical University, Tochigi, Japan b Division of Neurology, Jichi Medical University, Tochigi, Japan c Center for Gene and Cell Therapy, The Institute of Medical Science, The University of Tokyo, Japan ⁎ Corresponding author at: Dept. of Pediatrics, Jichi Me Shimotsuke-shi, Tochigi 329-0498, Japan.. E-mail address: hosaka@jichi.ac.jp (H. Osaka). http://dx.doi.org/10.1016/j.ymgme.2015.08.006 1096-7192/© 2015 Elsevier Inc. All rights reserved.. Please cite this article as: S. Nakamura, et al., (2015), http://dx.doi.org/10.1016/j.ymgme.2 a b s t r a c t a r t i c l e i n f o Article history: Received 8 July 2015 Received in revised form 9 August 2015 Accepted 9 August 2015 Available online xxxx Keywords: Glucose transporter I deficiency syndrome (GLUT1-DS) GLUT1 SLC2A1 Functional assay 2-deoxyglucose uptake Objective: We investigated a correlation between a mutation in the SLC2A1 gene and functional disorders in Glucose transporter I deficiency syndrome (GLUT1DS).



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    OriGene pcmv6 entry brk1 hspc300 myc ddk flag
    The interaction between Boc and the WRC occurs in live cells and is direct (A–D) The NanoBiT structural complementation reporter system was used to detect interaction between Boc and CYFIP2 in live cells. Boc was fused to the Large BiT (LgBiT) subunit (Boc-LgBiT), and CYFIP2 was fused to the Small BiT (SmBiT) subunit either N-terminally (CYFIP2-SmBiT) or C-terminally (SmBiT-CYFIP2) and expressed in HEK293 or COS7 cells. When the two proteins interact, a luminescent signal is generated in the presence of substrate. (A) Expression of Boc-LgBiT and CYFIP2-SmBit alone or (B) expression of Boc-LgBiT and SmBit-CYFIP2 alone does not generate a luminescent signal. (Left) When Boc-LgBiT is expressed with CYFIP2-SmBiT (A) or SmBit-CYFIP2 (B), they interact to generate a luminescent signal. (Right) Boc-LgBiT also interacts with CYFIP2-SmBiT (A) or SmBit-CYFIP2 (B) in the presence of co-expressed WRC components NCKAP1-Flag, WAVE1-Emerald, ABI1-EGFP, and <t>BRK1-Myc-Flag</t> (“+WRC”). Data are represented as mean ± SEM; error bars representing the SEM are too small to be visible. (Left) Repeated measures one-way ANOVA with Dunnett’s multiple comparison test, (right) paired t test. n = 2–6 independent experiments. ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001. (C) Boc lacking an intracellular domain (ICD), BocΔICD-LgBiT, has a significantly lower interaction with CYFIP2-SmBiT and (D) SmBiT-CYFIP2 compared to full-length Boc-LgBiT in the presence of the WRC components NCKAP1-Flag, WAVE1-Emerald, ABI1-EGFP, and BRK1-Myc-Flag. Data are represented as mean ± SEM. Paired t test, n = 3–5 independent experiments. ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001. (E) Schematic of GST-Boc ICD constructs. (F) Coomassie-blue-stained SDS-PAGE gel showing MBP pull-down between purified (MBP) 2 -tagged <t>HSPC300</t> or WRC and the indicated purified GST-Boc ICD proteins. Only GST-Boc ICD FL and GST-Boc ICD NT are pulled down by (MBP) 2 -WRC.
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    Image Search Results


    The interaction between Boc and the WRC occurs in live cells and is direct (A–D) The NanoBiT structural complementation reporter system was used to detect interaction between Boc and CYFIP2 in live cells. Boc was fused to the Large BiT (LgBiT) subunit (Boc-LgBiT), and CYFIP2 was fused to the Small BiT (SmBiT) subunit either N-terminally (CYFIP2-SmBiT) or C-terminally (SmBiT-CYFIP2) and expressed in HEK293 or COS7 cells. When the two proteins interact, a luminescent signal is generated in the presence of substrate. (A) Expression of Boc-LgBiT and CYFIP2-SmBit alone or (B) expression of Boc-LgBiT and SmBit-CYFIP2 alone does not generate a luminescent signal. (Left) When Boc-LgBiT is expressed with CYFIP2-SmBiT (A) or SmBit-CYFIP2 (B), they interact to generate a luminescent signal. (Right) Boc-LgBiT also interacts with CYFIP2-SmBiT (A) or SmBit-CYFIP2 (B) in the presence of co-expressed WRC components NCKAP1-Flag, WAVE1-Emerald, ABI1-EGFP, and BRK1-Myc-Flag (“+WRC”). Data are represented as mean ± SEM; error bars representing the SEM are too small to be visible. (Left) Repeated measures one-way ANOVA with Dunnett’s multiple comparison test, (right) paired t test. n = 2–6 independent experiments. ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001. (C) Boc lacking an intracellular domain (ICD), BocΔICD-LgBiT, has a significantly lower interaction with CYFIP2-SmBiT and (D) SmBiT-CYFIP2 compared to full-length Boc-LgBiT in the presence of the WRC components NCKAP1-Flag, WAVE1-Emerald, ABI1-EGFP, and BRK1-Myc-Flag. Data are represented as mean ± SEM. Paired t test, n = 3–5 independent experiments. ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001. (E) Schematic of GST-Boc ICD constructs. (F) Coomassie-blue-stained SDS-PAGE gel showing MBP pull-down between purified (MBP) 2 -tagged HSPC300 or WRC and the indicated purified GST-Boc ICD proteins. Only GST-Boc ICD FL and GST-Boc ICD NT are pulled down by (MBP) 2 -WRC.

    Journal: iScience

    Article Title: The WAVE regulatory complex interacts with Boc and is required for Shh-mediated axon guidance

    doi: 10.1016/j.isci.2024.111333

    Figure Lengend Snippet: The interaction between Boc and the WRC occurs in live cells and is direct (A–D) The NanoBiT structural complementation reporter system was used to detect interaction between Boc and CYFIP2 in live cells. Boc was fused to the Large BiT (LgBiT) subunit (Boc-LgBiT), and CYFIP2 was fused to the Small BiT (SmBiT) subunit either N-terminally (CYFIP2-SmBiT) or C-terminally (SmBiT-CYFIP2) and expressed in HEK293 or COS7 cells. When the two proteins interact, a luminescent signal is generated in the presence of substrate. (A) Expression of Boc-LgBiT and CYFIP2-SmBit alone or (B) expression of Boc-LgBiT and SmBit-CYFIP2 alone does not generate a luminescent signal. (Left) When Boc-LgBiT is expressed with CYFIP2-SmBiT (A) or SmBit-CYFIP2 (B), they interact to generate a luminescent signal. (Right) Boc-LgBiT also interacts with CYFIP2-SmBiT (A) or SmBit-CYFIP2 (B) in the presence of co-expressed WRC components NCKAP1-Flag, WAVE1-Emerald, ABI1-EGFP, and BRK1-Myc-Flag (“+WRC”). Data are represented as mean ± SEM; error bars representing the SEM are too small to be visible. (Left) Repeated measures one-way ANOVA with Dunnett’s multiple comparison test, (right) paired t test. n = 2–6 independent experiments. ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001. (C) Boc lacking an intracellular domain (ICD), BocΔICD-LgBiT, has a significantly lower interaction with CYFIP2-SmBiT and (D) SmBiT-CYFIP2 compared to full-length Boc-LgBiT in the presence of the WRC components NCKAP1-Flag, WAVE1-Emerald, ABI1-EGFP, and BRK1-Myc-Flag. Data are represented as mean ± SEM. Paired t test, n = 3–5 independent experiments. ∗∗ p < 0.01, ∗∗∗∗ p < 0.0001. (E) Schematic of GST-Boc ICD constructs. (F) Coomassie-blue-stained SDS-PAGE gel showing MBP pull-down between purified (MBP) 2 -tagged HSPC300 or WRC and the indicated purified GST-Boc ICD proteins. Only GST-Boc ICD FL and GST-Boc ICD NT are pulled down by (MBP) 2 -WRC.

    Article Snippet: pCMV6-Entry-BRK1(HSPC300)-Myc-DDK(Flag) , Origene , Cat# RC200804.

    Techniques: Generated, Expressing, Comparison, Construct, Staining, SDS Page, Purification

    Journal: iScience

    Article Title: The WAVE regulatory complex interacts with Boc and is required for Shh-mediated axon guidance

    doi: 10.1016/j.isci.2024.111333

    Figure Lengend Snippet:

    Article Snippet: pCMV6-Entry-BRK1(HSPC300)-Myc-DDK(Flag) , Origene , Cat# RC200804.

    Techniques: Virus, Recombinant, Activity Assay, Protease Inhibitor, Molecular Weight, Concentration Assay, Sequencing, Modification, Affinity Purification, Expressing, shRNA, Generated, Plasmid Preparation, Software, Blocking Assay